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Klebsiella pneumonia 1

Klebsiella pneumoniae 1

Antimicrobial resistance 1

Clinical mastitis 1

Fc fragment of the IgG-binding protein 1

Molecular characteristics 1

Population structure 1

children 1

computed tomography 1

liver abscess 1

mechanistic target of rapamycin kinase inhibitor 1

proteomics 1

severe Mycoplasma pneumoniae pneumonia 1

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Separation of 1,3-propanediol from glycerol-based fermentations of Klebsiella pneumoniae by alcohol precipitation

GAO Sujun, SUN Yaqin, XIU Zhilong

Frontiers of Chemical Science and Engineering 2007, Volume 1, Issue 2,   Pages 202-207 doi: 10.1007/s11705-007-0037-1

Abstract: The separation of 1,3-propanediol from the glycerol-based fermentation broth of plays an important role during the microbial production of 1,3-propanediol. In this paper, the separation of 1,3-propanediol from fermentative broth by a combination of ultrafiltration and alcohol dilution crystallization was investigated. The broth was first filtered by ultrafiltration, and 99% of cells, 89.4% of proteins and 69% of nucleic acids were removed. The obtained broth was further condensed by vac uum distillation, and then alcohol was added. The macromolecular impurities, such as nucleic acids, polysaccharides and proteins, were precipitated, and inorganic and organic salts were crystallized. The optimal volume ratio of alcohol added to the condensed fermentation broth was determined to be 2:1. As a result, proteins, nucleic acids and electric conductivity decreased by 97.4%, 89.7% and 95.8%, respectively, compared with the fermentative broth. The influences of pH and water content in condensed broth on alcohol precipitation and dilution crystallization were also investigated. The experimen tal results indicated that alcohol precipitation and dilution crystallization was feasible and effective for the separation of 1,3-propanediol from actual fermentation broth.
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Early diagnosis and therapeutic choice of

Jing LI, Ying FU, Ji-Yao WANG, Chuan-Tao TU, Xi-Zhong SHEN, Lei LI, Wei JIANG

Frontiers of Medicine 2010, Volume 4, Issue 3,   Pages 308-316 doi: 10.1007/s11684-010-0103-9

Abstract: Nowadays, pyogenic liver abscess (PLA) is still a common and severe intra-abdominal infection, and had emerged as the most common pathogenic bacteria worldwide in the past ten years. Our study aims to achieve an early pathogenic diagnosis and rational therapy modality for liver abscess (KLA) through clinical data analysis. A total of 197 inpatients in Zhongshan Hospital, Shanghai, diagnosed as having liver abscess between March 2001 and September 2009 were enrolled. Patients with monomicrobial infection were divided into two groups: patients with liver abscess (KLA group, =106) and those with non- liver abscess (NKLA group, =56). A retrospective analysis was made between these two groups on the aspects of underlying diseases, clinical characteristics, laboratory data, culture results, and imaging findings. To evaluate the effects of different medical interventions, monomicrobial KLA patients were further divided into four subgroups (percutaneous liver aspiration, aspiration plus antibiotics flushing, aspiration plus retained catheter, and aspiration plus antibiotics flushing and retained catheter), and corresponding therapeutic effects were analyzed. KLA was more likely to occur in patients with coexisting diseases such as diabetes mellitus (53.77% 25.00%, =0.001) and hepatic adipose infiltration (16.04% 5.36%, =0.029). Compared to NKLA group, clinical characteristics including abdominal pain (40.57% 57.14%, =0.044), hypodynamia (19.81% 46.43%, =0.001), and hepatomegaly (4.72% 14.29%, =0.033) were much milder, but with a higher fasting blood glucose level (7.84?±?0.36 5.76?±?0.30, =0.001) on admission in KLA group. In addition, KLA abscess often appeared singly in the right lobe of the liver with gas forming nature (32.88% 13.51%, =0.039), unsmooth rim (71.23% 40.54%, =0.002), and dynamic septum enhancement (41.10% 16.22%, =0.009). Compared to mono aspiration subgroup, additional antibiotic flushing could not further improve clinical outcomes of KLA patients ( >0.05); however, the retained catheter showed obvious advantage in reducing abscess diameter (34.38?±?3.25 mm 22.67?±?2.37 mm, =0.017). It can be concluded that the strong association with diabetes, milder clinical symptoms, and gas-forming nature in CT images makes early pathogenic diagnosis of KLA possible. Comparatively, ultrasonography-guided percutaneous liver aspiration with retained catheter may be the most rational intervention modality of KLA.

Keywords： liver abscess     Klebsiella pneumoniae     computed tomography     therapy    

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Molecular Epidemiology of Klebsiella pneumoniae from Clinical Bovine Mastitis in Northern Area Article

Shikai Song, Wenjuan He, Dawei Yang, Manar Benmouffok, Yao Wang, Jiyun Li, Chengtao Sun, Xiangbin Song, Shizhen Ma, Chang Cai, Shuangyang Ding, Congming Wu, Zhangqi Shen, Yang Wang,

Engineering 2022, Volume 10, Issue 3,   Pages 146-154 doi: 10.1016/j.eng.2021.01.015

Abstract:

Klebsiella pneumoniae (K. pneumonia, KpI) is a predominateHowever, data is lacking on the molecular characteristics of bovine K. pneumoniae, limitingLike human clinical K. pneumoniae, all CM K. pneumoniae isolates belonged to one ofthree phylogroups: KpI (n = 143), Klebsiella quasipneumoniae subsp.similipneumoniae (KpII-B) (n = 37), and Klebsiella variicola (KpIII) (

Keywords： Clinical mastitis     Klebsiella pneumonia     Molecular characteristics     Population structure     Antimicrobial    

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Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein

Frontiers of Medicine 2022, Volume 16, Issue 3,   Pages 378-388 doi: 10.1007/s11684-021-0840-y

Abstract: Macrolide and corticosteroid resistance has been reported in patients with Mycoplasma pneumoniae

Keywords： severe Mycoplasma pneumoniae pneumonia     children     proteomics     Fc fragment of the IgG-binding protein    

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Title Author Date Type Operation

Separation of 1,3-propanediol from glycerol-based fermentations of Klebsiella pneumoniae by alcohol precipitation

GAO Sujun, SUN Yaqin, XIU Zhilong

Journal Article

Early diagnosis and therapeutic choice of

Jing LI, Ying FU, Ji-Yao WANG, Chuan-Tao TU, Xi-Zhong SHEN, Lei LI, Wei JIANG

Journal Article

Molecular Epidemiology of Klebsiella pneumoniae from Clinical Bovine Mastitis in Northern Area

Shikai Song, Wenjuan He, Dawei Yang, Manar Benmouffok, Yao Wang, Jiyun Li, Chengtao Sun, Xiangbin Song, Shizhen Ma, Chang Cai, Shuangyang Ding, Congming Wu, Zhangqi Shen, Yang Wang,

Journal Article

Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein

Journal Article